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Recombinant Protein Expression, Purification and Characterization

Biologically active recombinant proteins are the lifeblood of the biotechnology industry. Once a gene has been cloned and its protein product expressed, it must be purified from the cell expression system. Further, the purified recombinant protein must characterized with regard to it physical and molecular properties. This course will feature state of the industry purification methods that are used to isolate purify, and characterize recombinant proteins. Hands-on laboratory exercises that employ the Green Fluorescent Protein (GFP) will used to showcase the methods used for protein purification (column chromatography) and characterization (polyacrylamide gel electrophoresis).

Students or employees who may benefit from this course include: laboratory technicians, chemists, molecular biologists, and others who need to understand the basic principles of protein expression, purification and characterization.